Abstract Background: The study of both intra- and interspecific competitions in Wheat (Triticum aestivum L.) agroecosystem is quite complex and it is essential to understand the influence of nitrogen in these competitions. Objective: The aim of this study was to evaluate the effect of different competitive models on nitrogen balance in wheat ecosystem agriculture (Chamran cultivar). Methods: A field experiment was performed using split-plot based on randomized complete block design (RCBD) with three replications. The main and sub-plots consisted of different nitrogen rates (control, 50, 100 and 150 kg nitrogen ha-1) and different competitive patterns (no, intraspecific, interspecific competition and intra- and interspecific in combination) were applied to the sub-plots, respectively. Weed density in the interspecies competition was applied through the equal planting of two narrow leaves (Avena sativa L.) and broadleaf (Sinapis arvensis L.) weed species. Results: The dry matter (DM) accumulation and nitrogen content (NC) at anthesis was higher than that at maturity stage for all competition treatments as well as nitrogen rates. The trend of nitrogen translocation efficiency (NTE) and nitrogen harvest index (NHI) was different from dry matter translocation efficiency (DMTE), and both were highest in interspecific competition treatments. Moreover, the highest grain yield in no competition treatment resulted in a lower dry matter and NTE. Conclusions: It was concluded that the capacity for nitrogen accumulation in the stem, was associated with a high nitrogen uptake, nitrogen assimilation and high post-anthesis nitrogen remobilization efficiency. In additions, high NHI could be used in the development of cultivars with the desired N balance.